Here is where we are located.
I’m moving the site from my own hand-coded html over to an instance of WordPress for 2015. There are several reasons why I am doing this:
I don’t seem to update too often if I hand code it making my lab webpage rather stale.
To dilute primers for PCR, you need to first make a 100µM stock solution. To accomplish this, you find the number of nMol on the tube and add 10X that much water in µl.
The key to the AFLP protocol is to be able to digest two restriction enzymes (RE’s) simultaneously and be able to ligate onto these sticky ends primers of known concentration. When you purchase new primers, you need to aliquot out usable volumes because repeated freeze/thaw cycles reduce RE efficiency.